Controlling selectivity-Solvent role-2.0
Lead This is in continuation to my earlier post <https://chiralpedia.com/blog/controlling-selectivity-solvent-role-1-0/> where we discussed the role solvent, first approach – varying solvent strength, in controlling retention factor and selectivity of chromatographic separation. In this post the focus is on the second approach, blending of solvents, to arrive at a good separation of difficult-to-separate peak pairs in HPLC method development. Second Approach: Blend solvents Òne can find suitable separation conditions for many samples simply by adjusting the …