Lead In the previous post, “Measuring quality of chromatogram – Tools 1.0” we talked about two of the separation attributes retention time and retention factor. In current post the discussion is on other features viz. peak asymmetry, peak shape, column plate number. and related aspects. This time let us try to answer the following queries …
Lead In the previous post, we talked about choosing suitable initial conditions when developing a new liquid chromatography (LC) method. The possibility of a successful separation is increased by selecting the appropriate column conditions and mobile phase composition. Getting the controls that quantitatively measure the separation’s quality is another crucial step in the method development …
Lead Starting out with a sound strategy is one of the most important aspects of minimizing chromatographic separation issues. A good method makes it much simpler to maintain operation within parameters and address issues as they appear. Selecting the right tools is very critical to achieve the best results,. By looking at the cause-and-effect diagram …
Lead Many chromatographers develop HPLC separations on a daily basis. The philosophy behind method development is founded on a number of factors. Today, chromatographic separation and how it varies depending on the sample and experimental conditions have a good practical understanding. This understanding of the chromatographic process should serve as the foundation for any methodological …
Lead Proteins are complex, high-MW biopolymers composed of L-amino acids and possess ordered 3D-structure. They are known to bind /interact stereoselectively with small molecules reversibly, making them extremely versatile CSPs for chiral separation of drug molecules. Number of CSPs has been developed by immobilizing proteins. These type of CSPs operate under RP-mode; (phosphate buffer …
Lead William H Pirkle developed these CSPs and are based on the ionic or covalent attachment of one enantiomer of an amino acid derivative to aminopropyl silica. Pirkle phases contain π-electron donor or acceptor rings substituted with H-bonding moieties. Donor–acceptor-type CSPs capitalize on synthetic or semi-synthetic chiral low-molecular-weight chiral selectors capable of recognizing chiral analytes …
Lead Cyclodextrin-based CSP belongs to the cavity type. Armstrong is considered the father of cyclodextrin-based CSPs. CDs are employed in various fields of analytical chemistry, due to their tendency to form reversible inclusion complexes and recognize chiral analytes. CDs are a type of macrocyclic D-(+)-glucopyranose oligomers linked by α-1,4-glycosidic linkages, as seen in Figure, under chemistry . …
Lead Surprisingly, chiral chromatography could not be performed in 1980 because there was no single chiral stationary phase on the market. But as the 1980s came to a close, chiral chromatography began to get more and more attention, thanks in large part to the efforts of Okamoto’s institute in Japan, Pirkle and Armstrong’s teams …
Lead A wide range of chiral compounds have been examined as chiral selectors with regard to their ability to separate enantiomers using chromatography as part of the development of CSPs. From low-molecular-weight chemicals to polymers with both synthetic and biological origins, the chiral molecules investigated as potential CSs span almost the full spectrum of chemical …
Chiral stationary phases (CSPs) The most convenient and most popular analytical methodology to assess chiral purity is the direct separation of enantiomers on so-called chiral stationary phases (CSPs). CSPs consists of an (ideally) inert chromatographic support ( usually silica micro-particles) on to which the chiral selector (CS; single enantiomer of a chiral molecule) is physically …