Introduction Chirality, or molecular handedness, plays a pivotal role in the chemistry of taste and smell. From the refreshing scent of mint to the sweet taste of artificial sweeteners, the chirality of molecules influences our sensory experiences in profound ways. In this blog, we will delve into the fascinating world of chiral compounds, exploring how …
The Chemistry of Taste and Smell: How Chirality Affects Senses Read More »
Introduction Chirality, derived from the Greek word for “hand,” is a property of asymmetry that is ubiquitous in nature. Just as our hands are mirror images but not identical, many biological molecules exhibit this fascinating characteristic. Chirality is not just a molecular curiosity; it profoundly influences the structure and function of DNA, proteins, and enzymes, …
Introduction Molecules can come in two shapes that mirror each other, in the same way as our left and right hands. This attribute, called chirality, can be found in biological molecules like sugars and proteins This property, often referred to as molecular handedness, plays a crucial role in chemistry, biology, and pharmacology. In this article, we …
Molecular Handedness: How Chirality Shapes Molecules Read More »
Lead Chirality, derived from the Greek word “cheir” meaning hand, is a fundamental concept in chemistry that describes an object’s property of being non-superimposable on its mirror image. This property is not just an abstract mathematical idea but has profound implications in various scientific fields, particularly chemistry and biology. Understanding chirality is essential for comprehending …
Introduction to Chirality: Understanding the Basics Read More »
Lead Changes in selectivity brought about by modifying the composition of the mobile phase have been discussed up until this point. This method is typically the most practical and economical way to alter selectivity. Alternately, relying on changes in column selectivity to control the separation is a strategy that is typically reserved until all other …
Controlling selectivity-Additional factors-3.0 – Column selectivity Read More »
Synopsis The development of chromatographic methods for the separation of chiral drugs presents a significant challenge, necessitating a comprehensive understanding of the compounds’ chemical properties and the factors influencing their separation. This task is further complicated by the presence of process-related impurities that can disrupt the separation process and compromise the quality of the results. …
Lead In the earliest posts we saw the how solvent concentration and type are powerful tools for controlling separation and also discussed these variables alone may not be sufficient for achieving the desired sample resolution. In such cases chromatographers may seek to employ other parameters to adjust selectivity viz.. In the last post the discussion …
Controlling selectivity-Additional factors-2.0 – temperature Read More »
Lead In the previous post the influence of solvent concentration and solvent type on the selectivity of liquid chromatography (LC) separations was discussed. We saw by systematically altering the solvent concentration, chromatographers can achieve dramatic changes in selectivity and retention. Changing the solvent type during method development, from acetonitrile to methanol, for instance, provided additional …
Controlling selectivity-Additional factors-1.0 – pH Read More »
Lead This is in continuation to my earlier post <https://chiralpedia.com/blog/controlling-selectivity-solvent-role-1-0/> where we discussed the role solvent, first approach – varying solvent strength, in controlling retention factor and selectivity of chromatographic separation. In this post the focus is on the second approach, blending of solvents, to arrive at a good separation of difficult-to-separate peak pairs in …
Lead Choosing a set of initial conditions that would be likely to produce a successful liquid chromatography (LC) separation was the main topic of discussion in the first post in the HPLC method development series. The majority of samples will benefit from starting with a C8 or C18 column packing based on Type B silica …