The Chemistry of Taste and Smell: How Chirality Affects Senses

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Introduction Chirality, or molecular handedness, plays a pivotal role in the chemistry of taste and smell. From the refreshing scent of mint to the sweet taste of artificial sweeteners, the chirality of molecules influences our sensory experiences in profound ways. In this blog, we will delve into the fascinating world of chiral compounds, exploring how they interact with our sensory receptors and shape the flavors and fragrances we encounter daily. By understanding the chemistry behind …

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Chirality in Nature: From DNA to Snail Shells

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Introduction Chirality, derived from the Greek word for “hand,” is a property of asymmetry that is ubiquitous in nature. Just as our hands are mirror images but not identical, many biological molecules exhibit this fascinating characteristic. Chirality is not just a molecular curiosity; it profoundly influences the structure and function of DNA, proteins, and enzymes, as well as various physiological and behavioral traits in plants and animals. In this blog, we will explore the role …

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Molecular Handedness: How Chirality Shapes Molecules

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Introduction Molecules can come in two shapes that mirror each other, in the same way as our left and right hands. This attribute, called chirality, can be found in biological molecules like sugars and proteins This property, often referred to as molecular handedness, plays a crucial role in chemistry, biology, and pharmacology. In this article, we will explore the basics of chirality, examine examples of chiral and achiral molecules, and delve into the methods used to …

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Introduction to Chirality: Understanding the Basics

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Lead Chirality, derived from the Greek word “cheir” meaning hand, is a fundamental concept in chemistry that describes an object’s property of being non-superimposable on its mirror image. This property is not just an abstract mathematical idea but has profound implications in various scientific fields, particularly chemistry and biology. Understanding chirality is essential for comprehending molecular interactions and their impacts on our daily lives. This first of this blog series delves into the definition, historical …

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Controlling selectivity-Additional factors-3.0 – Column selectivity

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Lead Changes in selectivity brought about by modifying the composition of the mobile phase have been discussed up until this point. This method is typically the most practical and economical way to alter selectivity. Alternately, relying on changes in column selectivity to control the separation is a strategy that is typically reserved until all other mobile phase alternatives have been exhausted. As with alterations to the selectivity of the mobile phase, chromatographers should make substantial …

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Harnessing JMP’s Prediction Profiler for Multi-Response Optimization of HPLC Method in Chiral Drug Separation

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Synopsis The development of chromatographic methods for the separation of chiral drugs presents a significant challenge, necessitating a comprehensive understanding of the compounds’ chemical properties and the factors influencing their separation. This task is further complicated by the presence of process-related impurities that can disrupt the separation process and compromise the quality of the results. However, the application of advanced statistical tools such as JMP’s Design of Experiments (DoE) and Prediction Profiler can significantly streamline …

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Controlling selectivity-Additional factors-2.0 – temperature

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Lead In the earliest posts we saw the how solvent concentration and type are powerful tools for controlling separation and also discussed these variables alone may not be sufficient for achieving the desired sample resolution. In such cases chromatographers may seek to employ other parameters to adjust selectivity viz.. In the last post the discussion was focused on the influence of pH on selectivity. In this blog the focus will be on the effect of …

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Controlling selectivity-Additional factors-1.0 – pH

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Lead In the previous post the influence of solvent concentration and solvent type on the selectivity of liquid chromatography (LC) separations was discussed. We saw by systematically altering the solvent concentration, chromatographers can achieve dramatic changes in selectivity and retention. Changing the solvent type during method development, from acetonitrile to methanol, for instance, provided additional selectivity leverage. In some instances, it is advantageous to combine multiple organic solvents. Although varying solvent concentration and type are …

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Controlling selectivity-Solvent role-2.0

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Lead This is in continuation to my earlier post <https://chiralpedia.com/blog/controlling-selectivity-solvent-role-1-0/> where we discussed the role solvent, first approach – varying solvent strength, in controlling retention factor and selectivity of chromatographic separation. In this post the focus is on the second approach, blending of solvents, to arrive at a good separation of difficult-to-separate peak pairs in HPLC method development. Second Approach: Blend solvents Òne can find suitable separation conditions for many samples simply by adjusting the …

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Controlling selectivity-Solvent role-1.0

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Lead Choosing a set of initial conditions that would be likely to produce a successful liquid chromatography (LC) separation was the main topic of discussion in the first post in the HPLC method development series. The majority of samples will benefit from starting with a C8 or C18 column packing based on Type B silica using a mobile phase of acetonitrile or methanol modified with water or a low-pH buffer, even though no single set …

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