HPLC Method Development – prelude
Lead Many chromatographers develop HPLC separations on a daily basis. The philosophy behind method development is founded on a number of factors. Today, chromatographic separation and how it varies depending on the sample and experimental conditions have a good practical understanding. This understanding of the chromatographic process should serve as the foundation for any methodological approach to HPLC method development. Most of the time, a desired separation can be easily accomplished with a minimal number …