Chiral stationary phases (CSPs) The most convenient and most popular analytical methodology to assess chiral purity is the direct separation of enantiomers on so-called chiral stationary phases (CSPs). CSPs consists of an (ideally) inert chromatographic support ( usually silica micro-particles) on to which the chiral selector (CS; single enantiomer of a chiral molecule) is physically coated (adsorption) or immobilization (covalently linked).   Basic concept The column is packed with a chiral selector (viz. polysaccharide or … Continue reading Direct chiral HPLC separation on CSPs