Strategy The basic aspect is to provide the right chiral environment where by the enantiomeric pair become distinguishable. In HPLC how do we construct the chiral environment? To understand this let us look at the variables available, in the HPLC system, that can be exploited for this purpose. The following is a schematic diagram of a typical HPLC system. The are three variables namely the analyte, mobile phase and stationary phase that can be alerted … Continue reading Chiral HPLC separation: strategy and approaches